Feast your eyes on the best microscope images of the year

From cricket tongues to DVD circuitry, an amazing world awaits under the microscope.

jumping spider eyes under a microscope
Jumping Spider Eyes
Jumping Spider EyesNoah Fram-Schwartz

Every year since 1974, Nikon has herded a gaggle of nerds into a room for an impossible task: Pore over thousands of microscope images and pick the very best ones.

I was lucky enough to join the judge's circle this summer for Nikon Small World 2014—the 40th year of the microscopy competition. Our small group had to peruse more than 1,200 entries from 79 countries.

It wasn’t easy. Many of the images had just the right mix of contrast and color to make them nearly leap off the screen. But our judging criteria went beyond visual allure; a gorgeous-looking image can be surprisingly commonplace while a mysterious-looking shot is borderline revelatory. So, we selected the finest shots based not only on appearance but also creativity, informational content, and technical execution.

The 20 images you’re about to see (including Mr. Jeepers Creepers jumping spider eyes, above) really are the best. There’s everything from psychedelic-looking algae and frighteningly detailed caterpillar feet to hyperrealistic rotifers and rainbow-colored worm babies.

One more thing before you dive in: If you find yourself curious about the techniques used to make these award-winning images, I'd recommend a visit to Molecular Expressions and MicroscopyU. These sites aren't exactly light reading, but they do host countless interactive visuals—including this animation, which demystifies the complex, Nobel Prize-winning superresolution microscopy method—that go a long way in helping you understand how the techniques work.

Click an image below to start blowing your mind.

crawling bone cancer (osteosarcoma)
Crawling Bone Cancer (20th Place)
This is the front part of a cell of bone cancer, or osteosarcoma, that's crawling using protein-based fibers called actin. The rainbow of color is added later to sort out the cell's complex structure. View the high-resolution version Technique: Structured illumination microscopy Magnification: 8000XDylan T. Burnette/Vanderbilt University School of Medicine
Larval stage of the acorn worm Balanoglossus misakiensis
Acorn Worm Larva (19th Place)
Shown here is an acorn worm larva, also known as Balanoglossus misakiensis, from above. The false color highlights cell borders, muscles, and even eye spots. View the high-resolution version Technique: Confocal microscopy Magnification: 10XSabrina Kaul/University of Vienna
scarlet pimpernel stamen
Scarlet Pimpernel (18th Place)
Famous as the symbol for Emma Orczy's turn-of-the-century novel, a scarlet pimpernel (or Anagallis arvensis) has a bundle of stamen that rises like a tower in this macro photograph. View the high-resolution version Technique: Macroscopy Magnification: 80XJens H. Petersen/MycoKey
Pleurotaenium ovatum micro algae
Micro Algae (17th Place)
Under polarized light, a tiny Pleurotaenium ovatum algae gives off dazzling colors. View the high-resolution version Technique: Polarized light, lambda plate Magnification: 40XRogelio Moreno
transgenic kidney cultures
Transgenic Kidneys (16th Place)
This extreme close-up shows three cultures of transgenic kidneys grown together. Red and blue-green colors highlight collisions of branching collecting duct systems. View the high-resolution version Technique: Confocal microscopy Magnification: 20XNilsNils Lindstrom/Developmental Biology, The Roslin Institute
jewel beetle exoskeleton
Jewel Beetle (15th Place)
The carapace near an eye of a jewel beetle, or Chrysochroa buqueti, throws off brilliant colors under a microscope. View the high-resolution version Technique: Diffused and reflected-illumination microscopy Magnification: 45XCharles Krebs/Charles Krebs Photography
fluorescent mouse brain
Mouse Brain (14th Place)
The vasculature of a mouse brain comes alive when the image is built up slice-by-slice using fluorescence. View the high-resolution version Technique: Light-sheet fluorescence microscopy Magnification: 2XAli Erturk
feeding Conochilus unicornis rotifers
Feeding Rotifers (13th Place)
These Conochilus unicornis rotifers, grouped into a colony, are actively feeding as they float around in water. View the high-resolution version Technique: Differential interference contrast Magnification: 417XCharles Krebs/Charles Krebs Photography
Montana Dryhead agate
Agate Mineral (12th Place)
An unpolished slab of Montana Dryhead agate reveals rich yellow and red minerals. View the high-resolution version Technique: Axial lighting microscopy Magnification: 50XDouglas Moore/University of Wisconsin - Stevens Point
microscopic cricket tongue
Cricket Tongue (11th Place)
No, this ain't a leaf: It's the tiny tongue of an Acheta domesticus house cricket. View the high-resolution version Technique: Rheinberg illumination (Dark field with interference filter) Magnification: 25XStefano Barone
daisy petal fungal infection
Infected Daisy (10th Place)
This false-color image of a daisy petal reveals a tiny, fuzzy, growing fungal infection near some pollen grains. View the high-resolution version Technique: Confocal autofluorescence Magnification: 10XPaul Joseph Rigby/CMCA, The University of Western Australia
parsley flower ovary
Parsley Ovary (9th Place)
The ovary of a parsley flower, or Petroselinum crispum, is stained to show lectin proteins (which bind sugars) in red and nuclei (which harbor DNA) in blue. View the high-resolution version Technique: Confocal laser scanning microscopy Magnification: 63XMeritxell Vendrell/Servei de Microscòpia, Universitat Autònoma
brine shrimp appendages under a microscope
Brine Shrimp Appendages (8th Place)
These feathery structures are actually the limbs of a infinitesimal common brine shrimp. View the high-resolution version Technique: Confocal microscopy Magnification: 100XIgor Robert Siwanowicz/Janelia Farm Research Campus, Howard Hughes Medical Institute
DVD player electronic circuit
DVD Player Circuitry (7th Place)
Electronic components are often too shiny to photograph, but polarized light can neutralize the glare. Shown here are a few parts of a DVD player's circuitry. View the high-resolution version Technique: Cross-polarized microscopy Magnification: 100XDennis Hinks
coral polyp flow
Coral Polyp Flow (6th Place)
Water and particles flow in and around the mouth of a hungry coral polyp (P. damicornis). View the high-resolution version Technique: Fluorescence and autofluorescence microscopy Magnification: 4XDouglas Brumley/Department of Civil and Environmental Engineering, Massachusetts Institute of Technology
cow artery cells
Cow Artery Cells (5th Place)
You're looking at a few pulmonary artery endothelial cells from a cow. They're stained for actin (pink), mitochondria (green), and DNA (yellow). View the high-resolution version Technique: Super-resolution microscopy Magnification: N/AMuthugapatti K. Kandasamy/Biomedical Microscopy Core, University of Georgia
hooked caterpillar foot
Caterpillar Foot (4th Place)
This terrifying orifice isn't the mouth of a beast, but rather the stubby little leg and foot of a caterpillar. The hooks reveal how the insect larvae manage to stick to almost any surface. (We dare you not to think of how scary a caterpillar's foot is the next time you pick one up.) View the high-resolution version Technique: Confocal and autofluorescence microscopy Magnification: 20XKarin Panser/Institute of Molecular Pathology
jumping spider eyes under a microscope
Jumping Spider Eyes (3rd Place)
This soul-chilling pair of eyes belongs to a common jumping spider. "I find that looking directly into a spider's front eyes is very powerful, as it's a perspective many of us aren't used to," says 19-year-old Noah Fram-Schwartz. Schwartz created this photo by splicing together 80 different microscope images, which have a very thin plane of focus, into one ultra-crisp image. View the high-resolution version Technique: Reflected light microscopy Magnification: 20XNoah Fram-Schwartz
calcite crystal under a microscope
Calcite Crystal (2nd Place)
Crystals love to grab and scatter light into a blinding glow under a microscope. But with a little polarized light, their angles and surface detail emerge in sharp relief. This is a piece of calcite, which is one of the most common minerals on Earth, yet occurs in a seemingly unlimited variety of shapes and colors. One thing all calcite crystals have in common, however, is perfect rhombohedral cleavage—a slanted-cube structure shown clearly in this image. View the high-resolution version Technique: Cross-polarized light microscopy Magnification: 10XAlessandro Da Mommio/Dipartimento di Scienze della Terra, Università di Pisa
Rotifer showing the mouth interior and heart-shaped corona
Swimming Rotifer (1st Place)
Although Rogelio Moreno of Panama only began his adventures in microscopy about five years ago, he's become a regular contender in the Nikon Small World competition. This year, however, he finally took first prize. Shown here is Moreno's rare shot of a high-speed rotifer caught open-mouthed and facing the camera, revealing its red, heart-shaped corona. Moreno babysat his microscope for hours on end for the right moment. When it came, he snapped a photo using a special beam-splitting strobe light—and froze this busy rotifer in place in sharp, almost 3-D–like relief. "Rotifers are very difficult to photograph," says Moreno. "I've always wanted to take this photo, but you have to wait and wait and wait." View the high-resolution version Technique: Differential interference contrast microscopy Magnification: 40XRogelio Moreno
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