Jeff submitted this photo, taken with a Nikon D80 at 180 sec, f/16, ISO 100. Two SB600 flashes were used; one in front from a 45-degree angle and one in back to separate the frog from the background. The subject matter is unique and I am amazed the frog stayed in place. The back light did as intended separating and defining the frog from behind, the front light could have used a snoot or a gobo, something to block it from hitting so much of the black velvet to give you a more uniformed black if that was your intent. I would suggest a wider aperture creating a more shallow depth of field as long as your shutter speed doesn't exceed the synch speed of your speedlights.